There isn’t a way to force KaleidaGraph to draw the variable or curve fit lines over top of the markers. When the plot is drawn, all of the variable and curve fit lines are drawn first, followed by the markers. If you want to have a line appear on top of the markers instead of behind them, create two separate plots and overlay them in the layout window.
KaleidaGraph is a thoughtfully designed graphing and data analysis application for research scientists, as well as for those in business and engineering fields. It produces publication-quality graphs, and easily converts the most complex data into a functional display.